Daisy Drive Systems


Who should decide whether, when, and how to alter the environment? It's a hard question, especially when the decision will impact people in many different communities or nations. Daisy drive systems may help by empowering local communities to make decisions concerning their own local environments without imposing them on anyone else. Our technical manuscript describing daisy drives is available on bioRxiv and Responsive Science.

Daisy-chain gene drives



The problem with current CRISPR-based gene drive systems is that they can spread indefinitely. That means they could potentially affect every population of the target species throughout the world. It's unclear how such 'global' drives can be safely tested, much less whether nations will ever agree to use them.


To return power to the hands of local communities, we devised a new form of drive system called a “daisy drive” that can only affect local environments. The trick was to teach DNA to count.

Global drive systems are global because they carry everything they need to spread. A single piece of DNA encodes the desired alteration, the CRISPR system, and instructions to cut the original DNA sequence. In cells that produce sperm or eggs, CRISPR cuts the original version, causing the drive construct to be copied in its place. All of the organism's offspring will inherit a copy, editing will happen again in the offspring, and the process repeats until the drive system has spread through most or all of the population.

In daisy drive systems, the CRISPR components are split up and scattered throughout the genome so that none of them can drive on its own. Though physically separated, they're functionally arranged in a linear daisy-chain: element C causes element B to drive, and element B causes element A to drive.

Element C doesn't drive, so its abundance is limited by the number of daisy drive organisms released. And since changes made by humans are costly to the organism, natural selection will gradually eliminate C from the population. That means B will initially increase in abundance, then decline and vanish. In turn, A will increase even more rapidly, but eventually will run out of B and disappear.

In other words, the elements of a daisy drive system are like booster stages of a genetic rocket: those at the base of the daisy-chain help lift the payload until they run out of fuel and are successively lost. Adding more links to the daisy chain will spread the payload to more organisms. Releasing a daisy drive organism with a five-element chain (E→D→C→B→A) is hundreds of times more effective than releasing one with only element A.


Best of all, daisy drive systems can do anything a global drive system can do - including directly suppressing target populations - and use the same mechanism. That means communities could decide to use daisy drives to solve local problems, which could build and once they're shown to be changes that could later be spread using a global drive.

We joined up with researchers at Harvard University to figure out what exactly how powerful daisy drives would be. Charleston Noble, a graduate student who works with Martin Nowak and George Church, created detailed models using assumptions based on the efficiency of current gene drive systems.

The research hasn't been published in a peer-reviewed journal, and only two-element daisy drive systems, called “split drives,” have been previously demonstrated. But we're adamant that gene drive research must be open and responsive, which means telling people about the idea before running experiments. Closed-door science is wildly inappropriate when a laboratory accident could directly affect people outside the lab. We first described CRISPR-based gene drive before performing experiments to set this example, and we will always detail what we're planning to do – including proposed safeguards – well before we do it.

The major risk posed by daisy drive is that a rare event will move DNA encoding a drive component from one element to another, thereby creating a 'daisy necklace' capable of global drive. Since this kind of recombination depends on similarity of DNA sequences, we designed dozens of variants of the CRISPR components to come up with a set that aren't similar to one another, then worked with George Church's lab at Harvard to identify those that still work well enough to use. We should now have enough diverse components to build stable 5-element drive systems.

Of course, with drive systems, 'should' isn't good enough. John Min, a graduate student in Sculpting Evolution, is now working to build daisy drive systems in nematode worms, which reproduce quickly and can be grown in the laboratory in the billions. The idea is to observe their evolution in extremely large populations – ideally, at least as many organisms as would be altered if a daisy drive system were to be released into the wild - in order to confirm that they are stable enough to consider deploying.

We hope that daisy drives will simplify decision-making and promote responsible use by allowing local communities to decide how to solve their own ecological problems.